Journal:

Article Title: Antigen Transport into Peyer's Patches

doi:

Figure Lengend Snippet: Quantitation of the uptake of latex microbeads in the flanks of the Peyer’s patch domes. S. pneumoniae R36a, R36A, or buffer only (controls) were instilled into the ileal lumen for 3 hours, and latex microbeads were instilled for the next 45 minutes. The median number of particles per M cell (thick line) is significantly increased (* P < 0.05) in the R36a- and R36A-treated patches, as compared to controls. The boxplot-whisker diagram shows that both bacterial strains up-regulate particle transport in the dome epithelium, and that this is due to an increased transport capacity of the M cells. N is the number of domes investigated per group. Boxes represent the lower and upper quartile values, and extreme values are displayed as whiskers.

Article Snippet: Stock cultures of S. pneumoniae R36a (rough phase; ATCC 27336) and R36A (smooth phase; ATCC 11733) were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Quantitation Assay, Whisker Assay

Journal:

Article Title: Antigen Transport into Peyer's Patches

doi:

Figure Lengend Snippet: a to c: Uptake of microbeads by M cells after instillation of S. pneumoniae R36a and R36A into the gut lumen for 3 hours. Fluorescent microbeads, 0.5-μm in diameter, are visible as bright dots in the green channel, which also contains the cytokeratin labeling (a to c). Three-color fluorescence of enterocytes, M cells and lymphocytes (a’ to c’) allows the flank region of the domes to be identified and the average number of particles per M cell to be determined quantitatively. The three images selected illustrate a low uptake rate (a and a’ in a control patch), a high rate (b and b’ in a R36a-treated patch), and a moderate particle uptake rate (c and c’ in a R36A-treated Peyer’s patch). d to f: The numbers of M-cell associated lymphocytes (some of which are marked by asterisks in d to f) and M cells (cytokeratin+ vimentin+, orange in d’ to f’) were determined for each flank so that the average number of lymphocytes per M-cell section profile could be calculated. The three images selected illustrate a low (d and d’), high (e and e’), and moderate number of lymphocytes in M-cell pockets (f and f’). Magnifications: a to c’, ×380; d to f’, ×570.

Article Snippet: Stock cultures of S. pneumoniae R36a (rough phase; ATCC 27336) and R36A (smooth phase; ATCC 11733) were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Labeling, Fluorescence, Control